Part of the Hallmarks of Aging series.
What–and why–are telomeres?
The chromosomes that store your genetic information are capped at each end by a telomere–a specific DNA sequence, repeated thousands of times. This sequence serves two purposes: it protects the coding regions of the chromosomes from damage, and it provides a “clock” that measures the age of the cell.
The protective function is relatively straightforward.
DNA’s building blocks are made to bond with each other, which as a side effect makes them good at bonding with other molecules. This is dangerous–two chromosomes could bond together, or a chromosome could bond to something else entirely. Cells prevent this by placing a protein “cap” on the end of chromosomes, kind of like a cap on a pen. But since this cap bonds to DNA just like an opposing DNA strand, using the base pairs, the ends of chromosomes must be identical: otherwise each chromosome would need its own cap, and a mutation in the end of the gene would leave a chromosome vulnerable to fraying. Having a sequence that does nothing but signal “this is the end of a chromosome” avoids this problem.
Additionally, DNA damage can spontaneously change one base pair to another. This is more likely on the exposed ends of a chromosome than in the protected middle. Damage in coding regions is hard to detect and fix, because the repair enzyme needs to “know” what the original base pair was. However, damage in a non-coding region is not only inherently lower impact, but in the case of a repeating sequence, easier to fix. You only need a single enzyme that says, in essence, “is this near the end of a chromosome? Does it look close to the telomere sequence? Make it the telomere sequence.”
And due to a quirk of the structure of DNA, every time a chromosome is copied, one copy is a little shorter than the parent. If a coding region was shortened, it could ruin the resulting protein. Telomeres act as “cruft” that can be removed without affecting function.
The “clock” function of telomeres, on the other hand, is somewhat more complicated.
Because they consist of repeats, it is easy to lengthen telomeres after they’re shortened by cell division with an enzyme called telomerase. But in all cells except sperm, egg and stem cells, the lengthening process either does not occur or replaces less than what was lost. In the end, telomeres become and stay shorter with every cell division. Once a telomere shrinks to a certain size, its cell will no longer divide and eventually enters cellular senescence.
This causes substantial of negative consequences. As cells die off due to damage or disease, there are no cells to replace them, leading to organ degeneration. Furthermore, long telomeres predict increased lifespan. So why not just keep dividing indefinitely?
While there are no clear answers to this question, one story is that a limit on cell division prevents cancer. Cancer cells divide many more times than regular cells, and a mechanism that inhibits that runaway division can stop cancer before it becomes a problem. Indeed, breaking the inhibition against dividing when telomeres are short appears to play a key role in a cell becoming malignant.
What can we do?
Many lifestyle choices associated with good health (healthy diet, exercise, meditation, etc) are also associated with not only long telomeres, but lengthened telomeres. Lengthening can be detected in as little as six weeks after a behavioral change.
Researchers are also investigating several experimental treatments to extend telomeres. These include:
- Inserting extra copies of the telomerase gene into the subject’s DNA. When done in mice this lengthened lifespan, however DNA insertion can cause unpredictable breaks in other genes. BioViva, a Seattle-based biotech, has started experimentation in humans.
- Increasing telomerase activity less directly. It is common for enzymes to require a cofactor–that is, a small molecule that is not the target of the enzyme, but increases the rate of reaction. RevGenetics claims (with promising but incomplete experimental backing) that cycloastragenol is a cofactor for telomerase. This means that introducing cycloastragenol into cells could increase telomerase activity and thus lengthen telomeres. While promising, the compound has only been studied in mice, where it increased health span but not lifespan. In contrast to direct injection of DNA, this method has the advantage of being more controllable.
- Injecting telomerase RNA into cells. Typically, genes are expressed by the pathway DNA -> RNA -> protein. By injecting RNA directly, scientists cause more telomerase to be produced, but only until the RNA degrades. Like cofactor supplementation, this avoids concerns with endless lengthening contributing to cancer and the damage caused by inserting new genes. This has been tested only in cultured cells.
Want more? You can find a selection of articles related to this hallmark here.